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Hermaphrodites of the nematode Caenorhabditis elegans produce both sperm and oocytes in the same germline. To investigate the process underlying spermatogenesis and oogenesis separately, we used a quantitative proteomics approach applied to two mutant worm lines fem-3 q20 and fem-1 hc17 developing only male and female germlines, respectively. We used stable isotopic labeling of whole animals by feeding them either 14 N or 15 N labeled Escherichia coli.

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Find more information on the Altmetric Attention Score and how the score is calculated. Hermaphrodites of the nematode Caenorhabditis elegans produce both sperm and oocytes in the same germline. To investigate the process underlying spermatogenesis and oogenesis separately, we used a quantitative proteomics approach applied to two mutant worm lines fem-3 q20 and fem-1 hc17 developing only male and female germlines, respectively.

We used stable isotopic labeling of whole animals by feeding them either 14 N or 15 N labeled Escherichia coli. This way, we could confidently identify and quantify proteins in two independent experiments.

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As expected, proteins linked to oogenesis were found to be highly upregulated in the feminized worms, whereas proteins involved in spermatogenesis were found to be highly upregulated in the masculinized worms. This was complemented by many proteins strongly enriched in either mutant. We show that members of several protein complexes as well as functionally similar proteins show comparable abundance ratios, indicating coregulation of protein expression.

Additional analysis comparing our protein data to a ly published microarray data set shows that mRNA and protein expression are poorly correlating.

Worms from venus and mars: proteomics profiling of sexual differences in caenorhabditis elegans using in vivo 15n isotope labeling

We provide one of the first examples of a large-scale quantitative proteomics experiment in C. Supplemental Figure 1, expression profiles of proteins belonging to the same protein complex or protein family. Supplemental Table 1, proteins and peptides that were identified and quantified in biological replicates, including label-swap experiments.

Supplemental Table 2, the relative abundance of all proteins identified and quantified in feminized and masculinized worms in the two independent label-swapped experiments.

Supplemental Table 3, the relative abundance of all proteins identified and quantified in feminized and masculinized worms in a single experiment. Supplemental Table 4, protein complexes and proteins with similar functionality quantified in fem-1 hc17 and fem-3 q20 animals. Such files may be downloaded by article for research use if there is a public use linked to the relevant article, that may permit other uses.

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Cited By. This article is cited by 13 publications. Gregory S. StuppChaevien S. ClendinenRamadan AjrediniMark A. SzewcTimothy GarrettRobert F. MengerRichard A. YostChris Beecherand Arthur S. Analytical Chemistry85 24 MeyerHannelore Danieland Britta Spanier.

Journal of Proteome Research11 9 ChikDavid C. SchriemerSarah J. Childsand James D. Proteome of the Caenorhabditis elegans Oocyte. Journal of Proteome Research10 5 Genes12 3 Combined transcriptome and proteome profiling reveals specific molecular brain atures for sex, maturation and circalunar clock phase.

Proteomics applications in Caenorhabditis elegans research. Biochemical and Biophysical Research Communications4 The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins.

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The application of quantification techniques in proteomics for biomedical research. Mass Spectrometry Reviews32 1 Elizabeth HulmeGeorge M. Angewandte Chemie21 Angewandte Chemie International Edition50 21 SchrimpfMichael O. A worm rich in protein: Quantitative, differential, and global proteomics in Caenorhabditis elegans.

Journal of Proteomics73 11 Pair your s. Your Mendeley pairing has expired.

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